F3040

Sigma

 

Monoclonal ANTI-FLAG® M1 antibody produced in mouse

clone M1, purified immunoglobulin, buffered aqueous solution

MDL number:MFCD00212941

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Description

ApplicationFor detection of amino-terminal FLAG fusion proteins by immunoprecipitation, immunoblotting, or EIA.
Biochem/physiol ActionsBinds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca2+-dependent; the complex dissociates in the absence of calcium ions.
Other NotesTo view FLAG® technology literature, visit www.sigma-aldrich.com/flagliterature.
Physical formSolution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).
Legal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
 FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

antibody formpurified immunoglobulin
cloneM1 (monoclonal)
formbuffered aqueous solution
application(s)immunoblotting (chemiluminescent): 10 μg/mL
isotypeIgG2b
shipped indry ice
storage temp.−20°C

Safety

WGK Germany3